Biological, chemical and combined treatments were used to degrade TBP. The biological treatment consisted in the use of Laetiporus sulphureus, Gloeophyllum trabeum and Ganoderma australe, which respectively achieved 48%, 74% and 80% degradation, and 40%, 70% and 77% of organic bromine removal (AOX) on TBP water solutions (60 mg L^-1) after 15 days of bio treatment. The biological treatment with G. australe on TBP-contaminated sawdust (10 mg kg^-1) led to 23% degradation. The chemical treatment consisted in the Hamilton system (Fenton reaction assisted by 1,2-dihydroxybenzene); observing with this treatment, 95% degradation, 50% mineralization (TOC), and 48% reduction of chemical oxygen demand (COD). The combined treatment of both (chemical-biological) treatments produced degradations of 100%, and reduction of toxicity of 34% and 30%, with L. sulphureus and G. australe, respectively.