• Log In
  • New issue alert
  • Submit a manuscript
  • Register
  • Home
  • About
  • Editorial Board
  • Search
  • Archives
  • Current
  • Forthcoming

Share

Article Panel


Vol 16, No 6 (2013)
»Table of Contents
Reading Tools
  • About the author
  • How to cite this article
  • Indexing metadata
  • Print version
  • Look up terms
  • Finding References
  • Review policy

Related items
  • Author's work


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International.
Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line | Crispo | Electronic Journal of Biotechnology
doi: 10.2225/vol16-issue6-fulltext-3
Electronic Journal of Biotechnology, Vol 16, No 6 (2013)

Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line

Martina Crispo, Geraldine Schlapp, Magdalena Cárdenas-Rodríguez, Dolores González-Maciel, Martín Rumbo



Abstract

Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize aspects of the procedure. Three different concentrations (0.5, 1.0 and 4.0 ng/µl) of the DNA construction to microinject a total of 1981 zygotes has been tested. Intact/injected embryos, pregnancy and birth rate, survival of pups 7 days after birth, number of transgenic pups and overall transgenic efficiency was registered and analyzed by Z test of proportions for each group.

Results: A total of seven transgenic founders were detected for the three DNA concentrations used, 1 in 46 alive pups in the 0.5 ng/ml group, 5 in 38 alive pups in the 1 ng/ml group and 1 in 21 alive pups in the 4 ng/ml group (p < 0.1). The overall transgenic efficiency was higher for the 1 ng/ml concentration, with a transgenic rate of 13.2%.

Conclusions: In conclusion, we have selected the best operative conditions to maximize the transgenesis efficiency. Furthermore, the transgenic lines developed could be used as a reporter model of innate immunity activation with many different applications in the fields of immunology, cancer and neurodegenerative diseases.




Full Text: | HTML | Reprint PDF | HTML

ISSN:  0717-3458

Contact: edbiotec@pucv.cl

Pontificia Universidad Católica de Valparaíso
Av. Brasil 2950, Valparaíso, Chile
Copyright © 1997- 2023 by Electronic Journal of Biotechnology