Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l^-1, pH 7.00).

Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H₂.l^-1, productivity 0.3167 mg H₂.h^-1 l^-1 culture mediumand yield 0.1962 mol H₂.mol^-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l^-1.h^-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R² = 0.95 for biomass substrate, R² = 0.77 hydrogen production).

Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.