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Vol 16, No 2 (2013)
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Evaluating Colombian Clostridium spp. strains’ hydrogen production using glycerol as substrate | Bernal | Electronic Journal of Biotechnology
doi: 10.2225/vol16-issue2-fulltext-4
Electronic Journal of Biotechnology, Vol 16, No 2 (2013)

Evaluating Colombian Clostridium spp. strains’ hydrogen production using glycerol as substrate

José Mauricio Bernal, Lizeth Katherine Tinoco Navarro, Luz Angela Torres Tacha, Dionisio Humberto Malagón, Dolly Montoya



Abstract

Background: The ability for hydrogen production of 13 native strains of Clostridium spp. strains isolated from Colombian soil was evaluated using glycerol substrate. Glycerol to hydrogen conversion was investigated using a batch fermentation reactor and industrial glycerol source (50 g.l-1, pH 7.00).

Results: The results were quantified regarding acids, hydrogen, biomass and solvent production. The selected strain gave good hydrogen over production output at 14.4 mmol H2.l-1, productivity 0.3167 mg H2.h-1 l-1 culture mediumand yield 0.1962 mol H2.mol-1 glycerol. A further fermentation assay a 4.0 liter batch reactor let to being 0.26 mg.l-1.h-1 after 18 hrs of fermentation. Logistic model, Luedeking-Piret model and Luedeking-Piret modified models were used for modeling changes in cell growth, hydrogen production and substrate consumption (Correlation coefficients R2 = 0.95 for biomass substrate, R2 = 0.77 hydrogen production).

Conclusions: Our results indicate that hydrogen production through glycerol bioconversion by native strains is possible.




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ISSN:  0717-3458

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