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Vol 14, No 1 (2011)
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Ethanol production from sweet sorghum juice under very high gravity conditions: Batch, repeated-batch and scale up fermentation | Nuanpeng | Electronic Journal of Biotechnology
doi: 10.2225/vol14-issue1-fulltext-2
Electronic Journal of Biotechnology, Vol 14, No 1 (2011)

Ethanol production from sweet sorghum juice under very high gravity conditions: Batch, repeated-batch and scale up fermentation

Sunan Nuanpeng, Lakkana Laopaiboon, Penjit Srinophakun, Preekamol Klanrit, Prasit Jaisil, Pattana Laopaiboon



Abstract

Batch ethanol fermentations from sweet sorghum juice by Saccharomyces cerevisiae NP 01 were carried out in a 500 ml air-locked Erlenmeyer flask under very high gravity (VHG) and static conditions. The maximum ethanol production efficiency was obtained when 9 g l-1 of yeast extract was supplemented to the juice. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) were 120.24 ± 1.35 g l-1, 3.01 ± 0.08 g l-1 h-1 and 0.49 ± 0.01, respectively. Scale up ethanol fermentation in a 5-litre bioreactor at an agitation rate of 100 rev min-1 revealed that P, Qp and Yp/s were 139.51 ± 0.11 g l-1, 3.49 ± 0.00 g l-1 h-1 and 0.49 ± 0.01, respectively, whereas lower P (119.53 ± 0.20 g l-1) and Qp (2.13 ± 0.01 g l-1 h-1) were obtained in a 50-litre bioreactor. In the repeated-batch fermentation in the 5-litre bioreactor with fill and drain volume of 50% of the working volume, lower P and Qp were observed in the subsequent batches. P in batch 2 to 8 ranged from 103.37 ± 0.28 to 109.53 ± 1.06 g l-1.




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ISSN:  0717-3458

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