doi: 10.2225/vol13-issue5-fulltext-18
Electronic Journal of Biotechnology, Vol 13, No 5 (2010)
Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Abstract
A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.