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Vol 13, No 4 (2010)
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Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast | Rywińska | Electronic Journal of Biotechnology
doi: 10.2225/vol13-issue4-fulltext-1
Electronic Journal of Biotechnology, Vol 13, No 4 (2010)

Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast

Anita Rywińska, Waldemar Rymowicz, Marta Marcinkiewicz



Abstract

In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The volumetric citric acid productivity was markedly higher (1.05 and 0.94 g∙L-1h-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7 strains, respectively) in the cultures containing 200 g∙L-1 of carbon source.




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ISSN:  0717-3458

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