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Vol 12, No 2 (2009)
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A rapid and cheap protocol for preparation of PCR templates in peanut | Tang Wang | Electronic Journal of Biotechnology
doi: 10.2225/vol12-issue2-fulltext-11
Electronic Journal of Biotechnology, Vol 12, No 2 (2009)

A rapid and cheap protocol for preparation of PCR templates in peanut

Chuan Tang Wang, Xiu Zhen Wang, Yue Yi Tang, Jian Cheng Zhang, Shan Lin Yu, Jian Zhi Xu, Zhen Min Bao



Abstract

This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.




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ISSN:  0717-3458

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