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Vol 10, No 2 (2007)
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Effects of temperature, pH and additives on the activity of tannase produced by Paecilomyces variotii | Battestin | Electronic Journal of Biotechnology
doi: 10.2225/vol10-issue2-fulltext-9
Electronic Journal of Biotechnology, Vol 10, No 2 (2007)

Effects of temperature, pH and additives on the activity of tannase produced by Paecilomyces variotii

Vania Battestin, Gabriela A. Macedo



Abstract

A biochemical characterization of the tannase from a Paecilomyces variotii strain isolated in São Paulo, Brazil was carried out. Paecilomyces variotii is a strain obtained from the screening of five hundred fungi that were tested for their production of tannase. The enzyme produced was partially purified using ammonium sulfate precipitation followed by ion exchange chromatography, diethylaminoethyl (DEAE)-Sepharose. Effects of temperature and pH on the activity of crude tannase crude and purified tannase was studied. Km was found to be 0.61 µmol and Vmax = 0.55 U/mL. Temperature of 40 to 65ºC and pH 4.5 to 6.5 were optimum for tannase activity and stability; it could find potential use in the food-processing industry. The effects of different inhibitors, surfactants and chelators on the enzyme activity were also studied.




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ISSN:  0717-3458

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