• Log In
  • New issue alert
  • Submit a manuscript
  • Register
  • Home
  • About
  • Editorial Board
  • Search
  • Archives
  • Current
  • Forthcoming

Share

Article Panel


Vol 24 (2016)
»Table of Contents
Reading Tools
  • About the author
  • How to cite this article
  • Indexing metadata
  • Print version
  • Look up terms
  • Finding References
  • Review policy

Related items
  • Author's work


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivatives 4.0 International.
Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions | Luo | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2016.09.002
Electronic Journal of Biotechnology, Vol 24 (2016)

Enhancement of botrallin and TMC-264 production in liquid culture of endophytic fungus Hyalodendriella sp. Ponipodef12 after treatments with metal ions

Haiyu Luo, Dan Xu, Rushan Xie, Xuping Zhang, Jian Wang, Xuejiao Dong, Daowan Lai, Ligang Zhou, Yang Liu



Abstract

Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e., Mn2 +, Na+, Mg2 +, Zn2 +, Cu2 +, Fe2 +, Fe3 + and Al3 +) on botrallin and TMC-264 production in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 were investigated.

Results: Three most effective metal ions (Zn2 +, Cu2 + and Mg2 +) along with their optimum concentrations were screened. The optimum addition time and concentrations of Zn2 +, Cu2 + and Mg2 + were further obtained respectively for improving botrallin and TMC-264 production. The combination effects of Zn2 +, Cu2 + and Mg2 + on the production of botrallin and TMC-264 by employing statistical method based on the central composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264, which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and 36.63 mg/L accordingly with the optimum concentrations of Zn2 + at 0.81 mmol/L, Cu2 + at 0.20 mmol/L, and Mg2 + at 0.13 mmol/L in medium.

Conclusion: The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an effective strategy.




Full Text: | Reprint PDF | HTML

ISSN:  0717-3458

Contact: edbiotec@pucv.cl

Pontificia Universidad Católica de Valparaíso
Av. Brasil 2950, Valparaíso, Chile
Copyright © 1997- 2022 by Electronic Journal of Biotechnology