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Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modelling of its fermentation in shake flasks | Makhdum Munawar | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2015.07.005
Electronic Journal of Biotechnology, Vol 19, No 1 (2016)

Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modelling of its fermentation in shake flasks

Khalil Munawar Makhdum Munawar, Khanom Simarani, Mohamad Suffian Mohamad Annuar



Abstract

Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling.

Results: The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7, 28°C, aeration surface-to-volume ratio of 0.4 × 106 m- 1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated components as described by Luedeking-Piret kinetic model.

Conclusion: The findings of this study provided add to the literature on key variables in for achieving good microbial growth and mcl-PHA production in shake flasks culture. In addition, suitable kinetic model to describe cultivation in this system was also presented.




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ISSN:  0717-3458

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