Plumbagin production by root cultures of Plumbago rosea
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Keywords

high yielding plant
medium manipulation
naphthoquinone
Plumbago indica

How to Cite

1.
Panichayupakaranant P, Tewtrakul S. Plumbagin production by root cultures of Plumbago rosea. Electron. J. Biotechnol. [Internet]. 2002 Dec. 15 [cited 2024 Nov. 24];5(3):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v5n3-4

Abstract

Root cultures of Plumbago rosea Linn. were established from young leaf explants on solid Gamborg's B5 (B5) medium supplemented with the combination of α-naphthalene acetic acid (NAA) and kinetin in the concentration ranges of 0.5-2.0 mg/l and 0.1-0.5 mg/l, respectively. The production of plumbagin, determined by TLC-densitometry was higher [0.016 ± 0.0030% dry weight (DW)] in cultured roots obtained from B5 medium supplemented with 1.0 mg/l NAA and 0.1 mg/l kinetin. Plant selection increased the plumbagin production to 0.129 ± 0.0139% DW, while variation of sucrose and nitrogen (as (NH4)2SO4) concentration in B5 media slightly increase the plumbagin synthesis to 0.023 ± 0.0017 and 0.020 ± 0.0015% DW, respectively.

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