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Abstract
The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage. Abbreviations: MS: Murashige and Skoog medium (1962); 2,4-D: 2,4-dichlorophenoxyacetic acid; BAP: Benzylaminopurine; NAA: Naphthaleneacetic acid; Picloram [Picloram acid (4-amino-3, 5, 6 trichloropicolinic acid)], DMA (Dimetilamine salts of 3,6-dichloro-2-methoxybenzoic acid); INTA: Instituto Nacional de Tecnología Agropecuaria.
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