How to Cite
Abstract
This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture.
Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".
The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.