Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
Vol. 15 No. 6 (2012), Short Communications
Vol. 15 No. 6 (2012)

Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)

Short Communications
Published 2012-10-23
Da Qiu Zhao
Yangzhou University
Chen Xia Han
Yangzhou University
Jin Tao Ge
Yangzhou University
Jun Tao
Yangzhou University
Full Text
Reprint PDF

Keywords

anthocyanin
expression
glucosyltransferase
herbaceous peony.

How to Cite

1.
Zhao DQ, Han CX, Ge JT, Tao J. Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.). Electron. J. Biotechnol. [Internet]. 2012 Oct. 23 [cited 2024 Nov. 21];15(6). Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v15n6-5
APA Chicago Harvard IEEE MLA Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering.
Full Text
Reprint PDF

Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".

The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.