Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
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Keywords

Argemone mexicana
benzylisoquinoline alkaloids
genetic transformation
neomycin phosphotransferase II

How to Cite

1.
Godoy-Hernández G, Avilés-Berzunza E, Carrillo-Pech M, Vázquez-Flota FA. Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.). Electron. J. Biotechnol. [Internet]. 2008 Jan. 15 [cited 2024 Dec. 22];11(1):0-. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v11n1-3

Abstract

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.

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