Low-alcohol light beer enriched with olive leaves extract: Cold mashing technique associated with interrupted fermentation in the brewing process

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Low-alcohol light beer enriched with olive leaves extract: Cold mashing technique associated with interrupted fermentation in the brewing process
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Keywords

Antioxidant
Bitterness
Brewing
Cold mash
Interrupted fermentation
Leaves extract
Light beer
Low-alcohol
Olive
Phenolics
Plant extract

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How to Cite

1.
Cappelin E, Meneguzzi D, Hendges DH, Oldoni TLC, Daltoé MLM, Marchioro MLK, da Cunha MAA. Low-alcohol light beer enriched with olive leaves extract: Cold mashing technique associated with interrupted fermentation in the brewing process. Electron. J. Biotechnol. [Internet]. 2023 Mar. 15 [cited 2024 Nov. 21];68:81-90. Available from: https://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/2364

Abstract

Background: Beer is the most consumed alcoholic beverage globally, and the demand for differentiated beers with peculiar characteristics has intensified among beer consumers, creating a significant market niche. In this study, we developed a low alcohol light craft beer enriched with olive leaf extract (Olea europaea L.). The cold mashing technique associated with interrupted fermentation was used in the mashing step. Different concentrations of olive leaf extract (0.5, 1.0 and 2.0%) were added at the maturation stage. The samples were characterized by physicochemical parameters, phenolic and polyphenolic content, bioactive compounds, antioxidant potential, and microbiological quality.

Results: The cold mash technique associated with interrupted fermentation provided a low-alcohol beer (≅1.3%). The bitterness dimension (19.0 to 23.2 IBU) and color (9–17 EBC) parameter were in accordance with the Beer Judge Certification Program (BJCP) for the American Blond Ale-style. The addition of the extract enriched the content of total phenolics (171.09 to 437.4 mg GAE/mL) and polyphenolic (221.4 to 729.0 mg/L). Coumaric, ferulic, and cinnamic phenolic acids were detected in appreciable amounts in the beers. Oleuropein was the major compound in the beverage and plant extract. After adding 2% extract, the ABTS and DPPH radical scavenging activity, as well as the ferric reduction power, increased in beers by 28.4%, 449.1%, and 120.5%, respectively.

Conclusions: The extract of O. europaea L. promoted the enrichment of low-alcohol beer samples with bioactive compounds and antioxidant potential. The results obtained indicated the potential use of O. europaea L. extract as a natural oxidant in other beverages and food products.

https://doi.org/10.1016/j.ejbt.2024.01.002
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References

CERVBRASIL. Associação Brasileira da Indústria da Cerveja 2023. [cited 2023 Feb 3]. Avaliable from: http://www.cervbrasil.org.br/novo_site/dados-do-setor/.

Dziedzi?ski M, Stachowiak B, Kobus-Cisowska J, et al. Supplementation of beer with Pinus sylvestris L. shoots extracts and its effect on fermentation, phenolic content, antioxidant activity and sensory profiles. Electron J Biotechnol 2023;63:10–7. https://doi.org/10.1016/j.ejbt.2023.01.001

Sindicato Nacional da Indústria da Cerveja. Vendas de cerveja crescem 7,7% em 2021 2023. [cited 2023 Feb 3]. Avaliable from: https://www.sindicerv.com.br/noticias/vendas-de-cerveja-crescem-77-em-2021/.

Research and Markets. Non-alcoholic beer global market report 2023. [cited 2023 Nov 3]. Avaliable from: https://www.researchandmarkets.com/report/low-alcohol-beer.

Nehra M. Non alcoholic beers: Review and methods. Madridge J Food Technol 2022;7(1):200–6. https://doi.org/10.18689/mjft-1000130

Salan?? LC, Coldea TE, Ignat MV, et al. Non-alcoholic and craft beer production and challenges. Processes 2020;8(11):1382. https://doi.org/10.3390/pr8111382

Muller C, Neves LE, Gomes L, et el. Processes for alcohol-free beer production: A review. Food Sci Technol 2020;40(2):273–81. https://doi.org/10.1590/fst.32318

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa No 65, de 10 de Dezembro de 2019. [cited 2023 Feb 3]. Available from: https://pesquisa.in.gov.br/imprensa/jsp/visualiza/index.jsp?data=11/12/2019&jornal=515&pagina=31&totalArquivos=217.

Dalberto G, da Rosa MR, Niemes JP, et al. Cold mash in brewing process: Optimization of innovative method for low-alcohol beer production. ACS Food Sci Technol 2021;1(3):374–81. https://doi.org/10.1021/acsfoodscitech.0c00099

Schöttke N, Rögener F. Cold mashing - Analysis and optimization of extraction processes at low temperatures in the brewing process. E3S Web Conf 2021;247:01036. https://doi.org/10.1051/e3sconf/202124701036

Iorizzo M, Coppola F, Letizia F, et al. Role of yeasts in the brewing process: Tradition and innovation. Processes 2021;9(5):839. https://doi.org/10.3390/pr9050839

Palomino-Vasco M, Rodríguez-Cáceres MI, Mora-Díez N. Discrimination based on commercial/craft origin and on lager/ale fermentation of undiluted Spanish beer samples: front-face excitation-emission matrices and chemometrics. J Food Compos Anal 2023;115:104946. https://doi.org/10.1016/j.jfca.2022.104946

Strong G, England K. Beer Judge Certification Program. 2021 Style Guidelines. Beer Style Guidelines. [cited 2023 Feb 3]. Available from: https://www.bjcp.org/bjcp-style-guidelines/.

Costa PMC da, Almeida ILML de, Bianchini A, et al. Blond Ale craft beer production with addition of pineapple pulp. J Exp Agric Int 2019;38(2):1–5. https://doi.org/10.9734/jeai/2019/v38i230294

Ambra R, Pastore G, Lucchetti S. The role of bioactive phenolic compounds on the impact of beer on health. Molecules 2021;26(2):486. https://doi.org/10.3390/molecules26020486 PMid: 33477637

Bertan FAB, da Silva Pereira Ronning E, Marchioro MLK, et al. Valorization of pineapple processing residues through acetification to produce specialty vinegars enriched with red-Jambo extract of Syzygium malaccense leaf. Sci Rep 2022;12:19384. https://doi.org/10.1038/s41598-022-23968-2 PMid: 36371484

González E, Gómez-Caravaca AM, Giménez B, et al. Evolution of the phenolic compounds profile of olive leaf extract encapsulated by spray-drying during in vitro gastrointestinal digestion. Food Chem 2019;279:40–8. https://doi.org/10.1016/j.foodchem.2018.11.127 PMid: 30611506

Ciont C, Difonzo G, Pasqualone A, et al. Phenolic profile of micro- and nano-encapsulated olive leaf extract in biscuits during in vitro gastrointestinal digestion. Food Chem 2023;428:136778. https://doi.org/10.1016/j.foodchem.2023.136778 PMid: 37421669

Alrugaibah M, Yagiz Y, Gu L. Novel natural deep eutectic solvents as efficient green reagents to extract phenolic compounds from olive leaves and predictive modelling by artificial neural networking. Food Bioprod Process 2023;138:198–208. https://doi.org/10.1016/j.fbp.2023.02.006.

Acar-Tek N, A?agündüz D. Olive leaf (Olea europaea L. folium): Potential effects on glycemia and lipidemia. Ann Nutr Metab 2020;76(1):10–5. https://doi.org/10.1159/000505508 PMid: 31901903

Menezes RCR, Peres KK, Costa-Valle MT, et al. Oral administration of oleuropein and olive leaf extract has cardioprotective effects in rodents: A systematic review. Rev Port Cardiol 2022;41(2):167–75. https://doi.org/10.1016/j.repc.2021.05.011 PMid: 36062705

ASBC. Beer methods. Am Soc Brew Chem 2023. [cited 2023 Feb 3]. Available from: https://www.asbcnet.org/Methods/BeerMethods/Pages/default.aspx.

Kanauchi M, Kultgen E, Bamforth C. Low-molecular-weight materials from heavily roasted barley and malt with strong foam-stabilising potential. J Inst Brew 2019;125(1):39–46. https://doi.org/10.1002/jib.538

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa No 161, de 1 de Julho de 2022. [cited 2023 Feb 3]. Available from: https://www.in.gov.br/en/web/dou/-/instrucao-normativa-in-n-161-de-1-de-julho-de-2022-413366880.

Oliveira CT de, Maia BHLNS, Ferriani AP, et al. Chemical characterization, antioxidant capacity and antimicrobial potential of essential oil from the leaves of Baccharis oreophila Malme. Chem Biodivers 2019;16(2):e1800372. https://doi.org/10.1002/cbdv.201800372 PMid: 30673172

Oldoni TLC, Santos S, Mitterer-Daltoé ML, et al. Moringa oleifera leaves from Brazil: Influence of seasonality, regrowth age and, region in biochemical markers and antioxidant potential. Arab J Chem 2022;15(11):104206. https://doi.org/10.1016/j.arabjc.2022.104206

Antonelo FA, Rodrigues MS, Cruz LC, et al. Bioactive compounds derived from Brazilian Myrtaceae species: Chemical composition and antioxidant, antimicrobial and cytotoxic activities. Biocatal Agric Biotechnol 2023;48:102629. https://doi.org/10.1016/j.bcab.2023.102629

Singleton, VL.; Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol 1999;299:152–78.

European Brewery Convention. Analytica-Microbiologica-EBC. 2 sd. Nürnberg: Fachverlag Hans Carl; 2005.

Iurckevicz G, Dahmer D, Santos VAQ, et al. Encapsulated microparticles of (1?6)-?-?-glucan containing extract of Baccharis dracunculifolia: Production and characterization. Molecules 2019;24(11):2099. https://doi.org/10.3390/molecules24112099 PMid: 31163607

Kobayashi M, Nagahisa K, Shimizu H, et al. Simultaneous control of apparent extract and volatile compounds concentrations in low-malt beer fermentation. Appl Microbiol Biotechnol 2006;73:549–58. https://doi.org/10.1007/s00253-006-0516-1 PMid: 16865344

Alves MM, Rosa MS, Santos PPA dos, et al. Artisanal beer production and evaluation adding rice flakes and soursop pulp (Annona muricata L.). Food Sci Technol 2020;40(2):545–9. https://doi.org/10.1590/fst.36119

Klimczak K, Cioch-Skoneczny M. Changes in beer bitterness level during the beer production process. Eur Food Res Technol 2023;249:13–22. https://doi.org/10.1007/s00217-022-04154-0

Zhang Y, Jia S, Zhang W. Predicting acetic acid content in the final beer using neural networks and support vector machine. J Inst Brew 2012;118(4):361–7. https://doi.org/10.1002/jib.50

Guglielmotti M, Passaghe P, Buiatti S. Use of olive (Olea europaea L.) leaves as beer ingredient, and their influence on beer chemical composition and antioxidant activity. J Food Sci 2020;85(8):2278–85. https://doi.org/10.1111/1750-3841.15318 PMid: 32652593

Zenit R, Rodríguez-Rodríguez J. The fluid mechanics of bubbly drinks. Phys Today 2018;71(11):44–50. https://doi.org/10.1063/PT.3.4069

Aliyari MA, Motahar SFS, Salami M, et al. Structural, functional, and anti-cancer properties of conjugates of quinoa protein isolate and olive leaf polyphenolic extract: Application in production of bread. Food Struct 2022;33:100292. https://doi.org/10.1016/j.foostr.2022.100292

Francesco G de, Bravi E, Sanarica E, et al. Effect of addition of different phenolic-rich extracts on beer flavour stability. Foods 2020;9(11):1638. https://doi.org/10.3390/foods9111638 PMid: 33182668

Mazengia G, Dessalegn E, Dessalegn T. Effect of Moringa stenopetala leaf extracts on the physicochemical characteristics and sensory properties of lagered beer. Food Sci Nutr 2022;10(2):507–14. https://doi.org/10.1002/fsn3.2672 PMid: 35154687

Ribas JCR, Lazzari A, Gonzalez LBF, et al. Bioactive compounds and antioxidant activity of leaves from olive trees grown in Paraná, Brazil. Pesqui Agropecuária Bras 2023;58:e03025. https://doi.org/10.1590/s1678-3921.pab2023.v58.03025

Lins PG, Pugine SMP, Scatolini AM, et al. In vitro antioxidant activity of olive leaf extract (Olea europaea L.) and its protective effect on oxidative damage in human erythrocytes. Heliyon 2018;4(9):e00805. https://doi.org/10.1016/j.heliyon.2018.e00805 PMid: 30255162

Savi A, Calegari MA, Calegari GC, et al. Bioactive compounds from Syzygium malaccense leaves: Optimization of the extraction process, biological and chemical characterization. Acta Sci Technol 2020;42(1):e46773. https://doi.org/10.4025/actascitechnol.v42i1.46773

Chigurupati S, Alharbi FS, Almahmoud S, et al. Molecular docking of phenolic compounds and screening of antioxidant and antidiabetic potential of Olea europaea L. Ethanolic leaves extract. Arab J Chem 2021;14(11):103422. https://doi.org/10.1016/j.arabjc.2021.103422

Romero-Márquez JM, Navarro-Hortal MD, Jiménez-Trigo V, et al. An oleuropein rich-olive (Olea europaea L.) leaf extract reduces ?-amyloid and tau proteotoxicity through regulation of oxidative- and heat shock-stress responses in Caenorhabditis elegans. Food Chem Toxicol 2022;162:112914. https://doi.org/10.1016/j.fct.2022.112914 PMid: 35276233

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