Statistical optimization of media components for antibiotic production in Streptomyces sp. CMSTAAHAL-3

Abstract

Background: Actinomycetes particularly the Streptomyces sp. derived from marine and coastal habitats are regarded as the main source of antibiotics. In the current study, to isolate the antibiotic-producing actinomycetes sediment samples were collected from Manakudy estuary, Kanyakumari District, Tamil Nadu.

Results: The isolates were selected based on the antagonistic activity; the highly active isolate was identified as Streptomyces sp. CMSTAAHL-3. The culture media for the antibiotic production was optimized by one variable at a time and confirmed by the zone of inhibition against tested pathogens such as Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa. Then by altering the four factors starch, urea, MgSO₄ and NaCl at five levels, statistical optimization of the media components was explored using Response Surface Methodology-Central Composite Design and found that the predicted antibiotic response was closely correlated with the experimentally one and was confirmed by the zone of inhibition.

Conclusions: The Streptomyces sp. CMSTAAHL-3 and its antibiotics may be of great use in the treatment of a variety of pathogens, and the ideal culture medium discovered in this experiment will be helpful for further research involving large-scale fermentation for the effective production of antibiotics.