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Abstract
Background: The analytic techniques of methylation-sensitive amplification polymorphism and high-performance liquid chromatography were employed to detect variation DNA methylation of mature leaves of Chrysanthemum during the floral transition induced by short-day (SD) treatment.
Results: The time to capitulum bud appearance and early blooming from the date of planting were significantly shorter than those of the control for both an early and late-flowering cultivar. Capitulum development of the early- flowering cultivar was significantly accelerated compared with that of the control, which was different with the late-flowering cultivar. DNA methylation percentage of leaves changed dynamically during flower development. For the early-flowering cultivar, DNA methylation was 42.2%-51.3% before capitulum bud appearance and 30.5%-44.5% after capitulum bud appearance. The respective DNA methylation percentages for the late-flowering cultivar were 43.5%-56% and 37.2%-44.9%.
Conclusions: Percentage DNA methylation of leaves of Chrysanthemum decreased significantly during floral development. The decline in DNA methylation was elevated in the early-flowering cultivar compared with that of the late- flowering cultivar.Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".
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