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Vol 15, No 4 (2012)
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Callus induction and plant regeneration of Ulex europaeus | Ramírez | Electronic Journal of Biotechnology
doi: 10.2225/vol15-issue4-fulltext-4
Electronic Journal of Biotechnology, Vol 15, No 4 (2012)

Callus induction and plant regeneration of Ulex europaeus

Ingrid Ramírez, Fernando Dorta, Alvaro Cuadros-Inostroza, Hugo Peña-Cortés



Abstract

A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2% sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2% sucrose) showed rapid growth and development of roots (100%). Rooted plantlets were successfully transferred to soil in pots containing a 3:1 mixture of soil and vermiculite.    




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ISSN:  0717-3458

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