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Vol 13, No 5 (2010)
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Improved growth model for two-stage continuous cultures of Lactobacillus helveticus | Bouguettoucha | Electronic Journal of Biotechnology
doi: 10.2225/vol13-issue5-fulltext-16
Electronic Journal of Biotechnology, Vol 13, No 5 (2010)

Improved growth model for two-stage continuous cultures of Lactobacillus helveticus

Abdallah Bouguettoucha, Béatrice Balannec, Saci Nacef, Abdeltif Amrane



Abstract

An unstructured model for growth and lactic acid production during two-stage continuous cultures of Lactobacillus helveticus was previously developed. The Verlhust model was considered to describe growth kinetics. Production models was based on modified Luedeking-Piret expressions involving an inhibitory effect for the first stage (seed culture) and a nutritional limitation effect for the second stage (culture). To account for the decrease of the biomass concentration observed in the second stage, the dilution rate Dc was replaced by an exponential term of the dilution rate in the growth and product relations. Contrarily to the previous model, the important decrease of the biomass concentration observed at steady state in the second stage at high dilution rates, namely close to wash out, was correctly described by the new model. It also proved to satisfactory describes production data and volumetric productivity.




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ISSN:  0717-3458

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