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Vol 24 (2016)
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Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii | Pan | Electronic Journal of Biotechnology
doi:10.1016/j.ejbt.2016.08.006
Electronic Journal of Biotechnology, Vol 24 (2016)

Strategy of oxygen transfer coefficient control on the L-erythrulose fermentation by newly isolated Gluconobacter kondonii

Long Pan, Ya-kun Fang, Pei Zhou, Kui-qi Jin, Gang Wang, Yu-peng Liu



Abstract

Background: The effect of diverse oxygen transfer coefficient on the l-erythrulose production from meso-erythritol by a newly isolated strain, Gluconobacter kondonii CGMCC8391 was investigated. In order to elucidate the effects of volumetric mass transfer coefficient (kLa) on the fermentations, baffled and unbaffled flask cultures, and fed-batch cultures were developed in present work.

Results: With the increase of the kLa value in the fed-batch culture, l-erythrulose concentration, productivity and yield were significantly improved, while cell growth was not the best in the high kLa. Thus, a two-stage oxygen supply control strategy was proposed, aimed at achieving high concentration and high productivity of l-erythrulose. During the first 12 h, kLa was controlled at 40.28 h-1 to obtain high value for cell growth, subsequently kLa was controlled at 86.31 h-1 to allow for high l-erythrulose accumulation.

Conclusions: Under optimal conditions, the l-erythrulose concentration, productivity, yield and DCW reached 207.9 ± 7.78 g/L, 6.50 g/L/h, 0.94 g/g, 2.68 ± 0.17 g/L, respectively. At the end of fermentation, the l-erythrulose concentration and productivity were higher than those in the previous similar reports.




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ISSN:  0717-3458

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