Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 5, Issue of October 15, 2006

Figure 2. Schematic diagram to illustrate the basis to understand how the primers have to be designed containing the sequence of the restriction enzyme at the 5' end and with the 3' end oriented so the polymerase will amplify the entire plasmid but excluding the region to be deleted.

Supported by UNESCO / MIRCEN network