
Figure 3. Currents through mSlo and mSloGFP channels from transfected COS7 cell membranes reconstituted into lipid bilyers.
A: The currentvoltage relationship of mSlo channels, with the solid line representing a linear fit of 8 single channel experiments (n=5 for each data point). The standard error of the mean (SEM) is less than the width of the points and the average channel conductance was 265 pS. B: Voltage dependence of a typical single mSlo channel. The solid line represents a least squares fit of the Boltzman distribution. C: Single channel current (mSlo) activation by free Calcium added to the internal side of the channel in 150 mM symmetrical KCl. The data was leastsquares fitted with the Hill equation (K_{D} = 13.9 m M, n = 2.9, V_{max} = 94). D: The currentvoltage relationship of mSloGFP channels, with the solid line representing a linear fit of 9 single channel experiments (n=5 for each data point, except for +60 mV, where n =1). The standard error of the mean (SEM) is less than the width of the points and the average channel conductance was 272 pS. E: Voltage dependence of a single mSloGFP channel in 150 mM symmetrical KCl. The solid line represents a least squares fit of the Boltzman distribution. F: Single channel current (mSloGFP) activation by free Calcium added to the internal side of the channel in 150 mM symmetrical KCl. The data was leastsquares fitted with the Hill equation (K_{D} = 11.2 m M, n = 2.5, V_{max} = 84).
