Fig. 2 The results blasted with Genbank database.
(a) The cDNA of hLIF obtained using the overlap PCR. The length of ORF of hLIF is 609 bp, which is correct. Even though the C become to T, the amino acid is unchanged, and that is the silent mutation.
(b) The resultant hLIF cDNA by RT-PCR. The figure indicated the amino acid and the ORF were changed because there existed the mutation.