Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 3, Issue of July 15, 2008
 

Table 2. Primers used for generating RAPDs in traditional and improved cultivars of rice from Pakistan.


Sl.
No.

Primer's
name

Sequence
(5'~3')

Amplified
Fragments
(a)

Polymorphic
fragments
(b)

Percent
polymorphism
(b/a x 100)

Fragment
size
(bp)

1

OPA-01

CAGGCCCTTC

5

3

60

350 - 2000

2

OPA-02

TGCCGAGCTG

5

4

80

400 - 1350

3

OPA-04

AATCGGGCTG

10

8

80

500 - 1600

4

OPA-05

AGGGGTCTTG

5

2

40

500 - 1500

5

OPA-07

GAAACGGGTG

8

8

100

300 - 1600

6

OPA-08

GTGACGTAGG

10

10

100

200 - 1800

7

OPA-09

GGGTAACGCC

8

7

88

450 - 1450

8

OPA-10

GTGATCGCAG

7

6

86

400 - 1000

9

OPA-11

CAATCGCCGT

6

6

100

350 - 900

10

OPA-12

TCGGCGATAG

6

6

100

400 - 1600

11

OPA-13

CAGCACCCAC

8

6

75

425 - 1400

12

OPA-15

TTCCGAACCC

4

3

75

400 - 1250

13

OPA-16

AGCCAGCGAA

5

5

100

500 - 1200

14

OPA-17

GACCGCTTGT

15

15

100

550 - 3000

15

OPA-18

AGGTGACCGT

11

10

91

400 - 1650

16

OPA-19

CAAACGTCGG

12

12

100

300 - 3000

17

OPA-20

GTTGCGATCC

12

11

92

550 - 4000

18

OPB-06

TGCTCTGCCC

6

5

83

650 - 3000

19

OPB-07

GGTGACGCAG

8

8

100

500 - 3000

20

OPB-08

GTCCACACGG

12

10

83

350 - 2300

21

OPB-11

GTAGACCCGT

9

9

100

500 - 2000

22

OPB-12

CCTTGACGCA

5

3

60

700 - 2000

23

OPB-13

TTCCCCCGCT

8

7

88

500 - 4000

24

OPB-17

AGGGAACGAG

16

15

94

350 - 3500

25

OPB-18

CCACAGCAGT

7

7

100

600 - 3100

Total

208

186

 

 

 

  Supported by UNESCO / MIRCEN network