Table 2. Primers used for generating RAPDs in traditional and improved cultivars of rice from Pakistan.

Sl.
No.

Primer's
name

Sequence
(5'~3')

Amplified
Fragments
(a)

Polymorphic
fragments
(b)

Percent
polymorphism
(b/a x 100)

Fragment
size
(bp)

1

OPA-01

CAGGCCCTTC

5

3

60

350 - 2000

2

OPA-02

TGCCGAGCTG

5

4

80

400 - 1350

3

OPA-04

AATCGGGCTG

10

8

80

500 - 1600

4

OPA-05

AGGGGTCTTG

5

2

40

500 - 1500

5

OPA-07

GAAACGGGTG

8

8

100

300 - 1600

6

OPA-08

GTGACGTAGG

10

10

100

200 - 1800

7

OPA-09

GGGTAACGCC

8

7

88

450 - 1450

8

OPA-10

GTGATCGCAG

7

6

86

400 - 1000

9

OPA-11

CAATCGCCGT

6

6

100

350 - 900

10

OPA-12

TCGGCGATAG

6

6

100

400 - 1600

11

OPA-13

CAGCACCCAC

8

6

75

425 - 1400

12

OPA-15

TTCCGAACCC

4

3

75

400 - 1250

13

OPA-16

AGCCAGCGAA

5

5

100

500 - 1200

14

OPA-17

GACCGCTTGT

15

15

100

550 - 3000

15

OPA-18

AGGTGACCGT

11

10

91

400 - 1650

16

OPA-19

CAAACGTCGG

12

12

100

300 - 3000

17

OPA-20

GTTGCGATCC

12

11

92

550 - 4000

18

OPB-06

TGCTCTGCCC

6

5

83

650 - 3000

19

OPB-07

GGTGACGCAG

8

8

100

500 - 3000

20

OPB-08

GTCCACACGG

12

10

83

350 - 2300

21

OPB-11

GTAGACCCGT

9

9

100

500 - 2000

22

OPB-12

CCTTGACGCA

5

3

60

700 - 2000

23

OPB-13

TTCCCCCGCT

8

7

88

500 - 4000

24

OPB-17

AGGGAACGAG

16

15

94

350 - 3500

25

OPB-18

CCACAGCAGT

7

7

100

600 - 3100

Total

208

186