Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 2, Issue of April 15, 2008
 


Figure 3. PCR profile of doublets. PCR of CECret-CECdir (left) and CECret-CECdir (right) generated by three-primer driven PCR reaction at different (10, 1, 0.1 μM) hybrid primer concentrations resolved through agarose (2%) gel electrophoresis. Lanes1,5: Molecular Weight Ladder (Winkler Ltda, Santiago, Chile), Lanes 2-4: CECdir-CECret at 10, 1 and 0.1 μM hybrid primer ratio, Lanes 6-8 CECret-CECdir at 10, 1 and 0.1 μM hybrid primer ratio, Lane 9: negative control. The faint band right below 100 bp corresponds to primer dimers.


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