Figure 2. Doublets
design and sequence.
(a) Three-primer driven PCR reaction with hybrid primer (red-green) and flanking primers (red or green). DNA
templates are in red and green boxes.
(b) CECdir-CECret (top panel) and
CECret-CECdir (bottom panel) doublets construction design:
using CECdir (in
black) and CECret (in blue) as templates. Sequences of hybrid
primers used for fusion are underlined in red. Initiation codon ATG is omitted from scheme.
(c) A scheme of the amino acid sequence of
doublets of cecropin CECdir-CECret (top panel) and CECret-CECdir (bottom panel) from amino terminal to carboxi terminal orientation. Positively
charged amino acids are marked by plus symbols (+). Initial methionine amino
acid is omitted from scheme.